Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 280, Issue 15, Pages 15195-15201Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M500254200
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Funding
- NHLBI NIH HHS [N01-HV-28183] Funding Source: Medline
- NIAID NIH HHS [AI 35304 R01] Funding Source: Medline
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A random peptide library was expressed on the surface of a mammalian cell by applying retroviral vectors. The random sequence was fused to the CCR5 chemokine receptor, which served as a scaffold to present the library at the cell surface. We used this library to isolate an epitope mimetope in a proof of principle system. This approach can become a tool for rapid creation of peptidic expression domains in a eukaryotic environment. Applications include the creation of decoys for receptors in cell-cell interactions, screening for molecules that drive ligand expression on target cells in two-cell interaction screens, among other utilities.
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