Journal
JOURNAL OF BIOTECHNOLOGY
Volume 117, Issue 1, Pages 73-82Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2005.01.002
Keywords
laccase; polyphenol oxidase; amperometric biosensors; fuel-cells; Marinomonas mediterranea
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The incorporation of fungal laccases into novel applications has been delayed mainly due to their intrinsic sensitivity towards halides and alkaline conditions. In order to explore new sources of enzymes we evaluated the multipotent polyphenol oxidase PPO1 from the marine bacterium Marinomonas mediterranea. Here we report that, in contrast to its fungal counterparts, PPO1 remained functional above neutral pH presenting high specificity for phenolic compounds, in particular for methoxyl- substituted mono-phenols and catechols. These properties, in addition to its tolerance towards chloride (up to I M) and its elevated redox potential at neutral pH (0.9 V), suggest this enzyme may be an interesting candidate for specific applications such as the Amperometric determination of phenolic compounds and bio-fuel cells. (c) 2005 Elsevier B.V. All rights reserved.
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