Journal
CELL
Volume 121, Issue 2, Pages 271-280Publisher
CELL PRESS
DOI: 10.1016/j.cell.2005.02.019
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Funding
- NIDCR NIH HHS [DE10877, DE14721] Funding Source: Medline
- NIDDK NIH HHS [P30DK34854] Funding Source: Medline
- NIGMS NIH HHS [GM56203, R01 GM056203] Funding Source: Medline
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In eukaryotic cells, the SH2 and PTB domains mediate protein-protein interactions by recognizing phosphotyrosine residues on target proteins. Here we make the unexpected finding that the C2 domain of PKC delta directly binds to phosphotyrosine peptides in a sequence-specific manner. We provide evidence that this domain mediates PKC delta interaction with a Src binding glycoprotein, CDCP1. The crystal structure of the PKC delta C2 domain in complex with an optimal phosphopeptide reveals a new mode of phosphotyrosine binding in which the phosphotyrosine moiety forms a ring-stacking interaction with a histidine residue of the C2 domain. This is also the first example of a protein Ser/Thr kinase containing a domain that binds phosphotyrosine.
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