Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 818, Issue 2, Pages 285-291Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2005.01.010
Keywords
solid-phase microextraction; ibuprofen enantiomers; enantiomeric separation; urine
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A simple and rapid solid-phase microextraction method was developed for the enantioselective analysis of ibuprofen in urine. The sampling was made with a polydimethylsiloxane-divinylbenzene coated fiber immersed in the liquid sample. After desorptioning from the fiber, ibuprofen enantiomers were analyzed by HPLC using a Chiralpak AD-RH column and UV detection. The mobile phase was made of methanol-pH 3.0 phosphoric acid solution (75:25. v/v), at a flow rate of 0.45 mL/min. The mean recoveries of SPME were 19.8 and 19.1% for (-)-R-ibuprofen and (+)-(S)-ibuprofen, respectively. The method was linear at the range of 0.25-25 mu g/mL. Within-day and between-day assay precision and accuracy were below 15% for both ibuprofen enantiomers at concentrations of 0.75, 7.5 and 20 mu g/mL. The method was tested with urine quality control samples and human urine fractions after administration of 200 mg rac-ibuprofen. (c) 2005 Elsevier B.V. All rights reserved.
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