Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 17, Pages 6108-6113Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0502270102
Keywords
cAMP; G protein; Schizosaccharomyces pombe
Categories
Funding
- NIGMS NIH HHS [R01 GM046226-10A1, R01 GM046226, R01 GM046226-12, R01 GM046226-13, R01 GM046226-09, R01 GM046226-11, GM46226, F32 GM068399, GM068399, R01 GM046226-13S1] Funding Source: Medline
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G protein-mediated signaling is implicated in yeast and fungal cAMP pathways. By two-hybrid screens and pull-down experiments, we show that the fission yeast Gpa2 G alpha binds an N-terminal domain of adenylate cyclase, comprising a moderately conserved sequence within a region otherwise poorly related to other fungal adenylate cyclases. Overexpressing this domain in yeast perturbs cAMP signaling, which is restored by Gpa2 coexpression. Mutations affecting this domain, over 1,100 residues from the catalytic domain, alter glucose-triggered cAMP signaling. This is evidence for direct activation of adenylate cyclase by a fungal G protein and suggests a distinct activation mechanism from that of mammals.
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