Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 348, Issue 2, Pages 253-264Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2005.02.065
Keywords
Arabidopsis thaliana; ethylene signaling; transcription factor; DNA-binding domain; NMR; solution structure
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Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The ElN3/ElL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an ElN3/ElL protein, Arabidopsis thaliana ElL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy The structure consists of five g.-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding. (c) 2005 Elsevier Ltd. All rights reserved.
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