Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 106, Issue 1, Pages 383-387Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2004.08.021
Keywords
surface plasmon; optical waveguide; dual LEDs; differential measurement; immunoreaction
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Surface plasmon resonance (SPR) has been widely utilized for immunoreaction measurements. In this paper, we describe a novel SPR sensor with an optical waveguide and dual light emitting diodes (LEDs). The advantage of the system is that the refractive index of the waveguide and the wavelength of the LED can easily be changed. Also, it is suitable for the immobilization of antibodies and adsorption of molecules, since the waveguide has a planar structure. High sensitivity was realized by using a combination of two monochromatic light sources and so the refractive index range of the measured samples could be considered. The developed system is applied for measuring an immunoreaction. The results indicate that the proposed SPR system with dual LEDs is effective for biosensor applications. (c) 2004 Elsevier B.V. All rights reserved.
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