Enhancement of inosine production by Bacillus subtilis through suppression of carbon overflow by sodium citrate

Sodium citrate, added to culture medium at 2 g l(-1), increased inosine production by B. subtilis by 18% to 16 g l(-1). The phosphoenolpyruvate (PEP) pool was increased by 2.3-fold, while the pyruvate and acetate pools were decreased by 78% and 57%, respectively. Pyruvate kinase might thus be a regulatory site for inosine synthesis.