Relative retention order of all isomers of cis/trans conjugated linoleic acid FAME from the 6,8- to 13,15-positions using silver ion HPLC with two elution systems

CLA, defined as. one,or more octaclecadienoic. acids (18:2) with conjugated double bonds, has been reported to be active in a number of biological systems. GC and silver ion HPLC (Ag+-HPLC) have been the primary techniques for identifying specific CLA isomers in both foods and biological extracts. Recently, GC relative retention times were reported for all c c, c/t (c,t and t,c), and t,t CLA FAME from the 6,8- to the 13,1 5-positions in octadecadienoic acid (18:2). Presented here is the rela-, live retention order of the same CLA FAME using Ag+-HPLC with two different elution systems. The first elution system, consisting of 0.1 % acetonitrile/0.5`/` diethyl ether (DE) /hexane, has been used previously to monitor CLA composition in foods. Also presented here is the retention order of CLA FAME using 2%. acetic acid/hexane elution solvent', which has advantages of more stable retention volumes and a complementary elution order of CLA FAME isomers. The data are reported using retention volumes (RV) adjusted for toluene, an estimator for dead volume, and relative to c9,t1 1-18:2. Measurement of relative RV in the analysis of 88 samples of cow plasma, milk, and rumen fluids using Ag+-HPLC is also presented here. The % CV ranged from 1.04 to 1.62 for t, t isomers and from 0 to 0.48 for c/t isomers.