Journal
HUMAN VACCINES
Volume 1, Issue 3, Pages 123-128Publisher
LANDES BIOSCIENCE
DOI: 10.4161/hv.1.3.1924
Keywords
Ancylostoma; Necator; hookworm; rat; ASP-2; vaccine; proliferation; antibody; cytokine
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Funding
- Human Hookworm Vaccine Initiative of the Sabin Vaccine Institute through Bill and Melinda Gates Foundation
- Human Hookworm Vaccine Initiative of the Sabin Vaccine Institute through Sabin Vaccine Institute
- International Research Scientist Development Award [1K01 TW00009]
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The recombinant larval protein Na-ASP-2 from the human helminth parasite Necator americanus is a lead candidate for a human hookworm vaccine. We have characterized the humoral and cellular immune responses elicited by the Na-ASP-2 formulated with the aluminum-based adjuvant Alhydrogel (R) in the Sprague Dawley rat. We demonstrated that Na-ASP-2 vaccine induced a strong antibody response at all doses tested, as evidenced by high specific IgG1, IgG2a and IgM titers. High IgG antibody titers were maintained up to three months post vaccination and were boosted by an additional vaccine dose. Specific cell proliferation and a Th2 cytokine profile were also observed in peripheral blood of all rats immunized with the formulated vaccine. Host IL-6 levels were also significantly elevated. These data provide evidence that the immune response of the Na-ASP-2 vaccine is robust and durable and therefore suitable for the further clinical development of the Na-ASP-2 vaccine.
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