Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 19, Issue 4, Pages 329-333Publisher
WILEY
DOI: 10.1002/bmc.444
Keywords
cobalamin; vitamin B-12; HPLC/radioisotope dilution assay
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A method has been developed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to routinely estimate the distribution of corrinoids (the cobalamins hydroxocobalamin, methylcobalamin and 5'-deoxyadenosylcobalamin, and cobalamin analogues) in liver, plasma, milk, intestinal fluid and faeces. Corrinoids were extracted with a sodium acetate buffer, separated by HPLC and quantified by RIDA. Recoveries of corrinoids were 29% for hydroxocobalamin, 50% for 5'-deoxyadenosylcobalamin and 64% for methylcobalamin. The method allows the routine analysis of many samples and maintains good standards of precision. Copyright (c) 2005 John Wiley & Sons, Ltd.
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