Journal
PEST MANAGEMENT SCIENCE
Volume 61, Issue 5, Pages 511-514Publisher
WILEY
DOI: 10.1002/ps.987
Keywords
tobacco; imidacloprid; residue; HPLC
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A practical method for analysis of residues of imidacloprid in baked tobacco leaves has been developed using HPLC with liquid-liquid partition clean-up. Imidacloprid was extracted with ethyl acetate under ultrasound, and cleaned up by liquid-liquid partition with 50 g litre(-1) aqueous sodium chloride followed by dichloromethane. The water-soluble and fat-soluble components in tobacco, which interfere with imidacloprid in HPLC, were removed. The separation was performed on a Supelco LC-18 column (250 mm. x 4.6mm ID) with a mobile phase of acetonitrile + 5mm ammonium acetate (20 + 80 by volume) at a flow-rate of 1 ml min(-1). The baseline separation between imidacloprid and the tobacco blank was achieved within 10 min. With a detection wavelength at 270 nm, the limit of quantitation was 0.04 mg kg(-1). The recovery ranged from 89.8 to 95.4 % and the RSDs were less than 2.3 %. The proposed method was successfully employed for the determination of imidacloprid residues in 300 samples of flue-cured tobacco leaves. (c) 2004 Society of Chemical Industry
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