Journal
BIOTECHNOLOGY ADVANCES
Volume 23, Issue 3, Pages 177-202Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2004.11.003
Keywords
recombinant proteins; Escherichia coli; secretion; periplasm; type II
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The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a reduced process cost. This review presents several strategies that can be used for recombinant protein secretion in E. coli and discusses their advantages and limitations depending on the characteristics of the target protein to be produced. (C) 2004 Elsevier Inc. All rights reserved.
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