Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 61, Issue 2, Pages 285-288Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2004.11.018
Keywords
contamination; real time PCR; universal PCR; platelet concentrates
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Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained. (C) 2004 Elsevier B.V. All rights reserved.
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