Journal
MEMORIAS DO INSTITUTO OSWALDO CRUZ
Volume 100, Issue 3, Pages 309-310Publisher
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762005000300017
Keywords
Leishmania (Viannia) braziliensis; Leishmania (Leishmania) amazonensis; replication rates; in vitro culture
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We compared the in vitro growth of promastigotes from two Leishmania species in TC-100 and Schneider media. Leishmania (Leishmania) amazonensis replication rates were similar in both tissue culture media and reached maximum rates by 48 h. In contrast Leishmania, (Viannia) braziliensis growth was significantly greater in TC-100 but maximum rates were achieved by 96 h. Folic acid appears to be the limiting factor and supplementation of Schneider media with this nutrient improved L. (V.) braziliensis replication rates and decreased the time of maximum replication to 48 h.
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