Journal
BLOOD
Volume 105, Issue 9, Pages 3577-3582Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2004-08-2980
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- NIGMS NIH HHS [GM57573-06] Funding Source: Medline
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Interaction between target cell CD47 and the inhibitory macrophage receptor signal regulatory protein a (SIRPa) counteracts macrophage phagocytosis of CD47-expressing host cells. As platelets also express CD47, we asked whether inhibitory CD47/SIRP alpha signaling regulates normal platelet turnover and clearance of platelets in immune thrombocytopenic purpura (ITP). CD47(-/-) mice had a mild spontaneous thrombocytopenia, which was not due to a decreased platelet half-life as a result of increased expression of P-selectin, CD61, or phosphatidylserine. In contrast, CD47(-/-) platelets were rapidly cleared when transfused into CD47(+/+) recipients, whereas CD47(+/-) platelets had a nearly normal half-life in CD47(+/+) mice under nonautoimmune conditions. CD47(-/-) mice were more sensitive to ITP, as compared with CD47(+/+) mice. In vitro, macrophage phagocytosis of immunoglobulin G (IgG)-opsonized CD47(-/-) platelets was significantly higher than that for equally opsonized CD47(+/+) platelets. However, when SIRP alpha was blocked, phagocytosis of CD47(+/+) platelets increased to the level of CD47(-/-) platelets. Phagocytosis of opsonized CD47(+/-) platelets was higher than that for CD47(+/+) platelets, but lower than that for CD47(-/-) platelets, suggesting a gene-dose effect of CD47 in this system. In conclusion, we suggest that inhibitory CD47/SIRP alpha signaling is involved in regulating platelet phagocytosis in ITP, and that targeting SIRP alpha may be a new means of reducing platelet clearance in ITR (c) 2005 by The American Society of Hematology.
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