Journal
JOURNAL OF ORTHOPAEDIC RESEARCH
Volume 23, Issue 3, Pages 646-652Publisher
WILEY
DOI: 10.1016/j.orthres.2004.09.007
Keywords
ultrasound; BMP-2; gene expression; stromal cells; osteogenesis
Categories
Ask authors/readers for more resources
To determine how low intensity pulsed ultrasound alters gene expression in rat bone marrow stromal cells and to see if combining this stimulation with BMP-2, cells were pre-cultured for eight days in the presence of 50 mu g/ml ascorbic acid and then exposed to either low intensity US or 100ng/ml BMP-2 or both combined, beginning on the first third fifth or seventh day Of Culture so that cells were exposed to the stimuli for one, three, five or seven days. Real time PCR was used to determine the effect of these treatmerits on gene expression of several genes associated with osteogenesis. The expression of some of the genes (Cbfa-1/Runx2, IGF-receptor, Alk-3, alkaline phosphatase, osteopontin, TGF-beta 1, BMP-7) was increased compared to untreated controls. Combination of US and BMP-2 treatment did not lead to synergy of the two stimuli. Cbfa-1 stimulation occurred more quickly with US than with BMP-2. Increases in gene expression were greatest after 3 days exposure to US, with similar results for BMP-2 treatment implying that there may be a time dependence for the stimulus of osteogenic gene expression in stromal cells. (c) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available