Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 61, Issue 7, Pages 1395-1401Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2004.10.048
Keywords
non-destructive analyses by NIR-FT-Raman spectroscopy; distribution of natural compounds in plants; library of IR and Raman spectra of natural compounds of plants; application in breeding; cultivation and quality control
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Ferdinand Bohlmann has described the isolation, the identification and the structure elucidation of acetylene compounds in many plants, and confirmed it by its synthesis. We have recorded the Raman spectra of most of these plants non-destructively by FT-Raman spectroscopy using radiation at 1064 nm. We could not observe any interfering fluorescence. We found acetylene compounds in some plants, even distinct compounds with different concentration in various parts of it. The distribution of the different compounds over the plant can be observed and their changes during the ontogenesis can be followed by a FT-Raman mapping technique. Of special help is a library of Raman and IR spectra and the structure of the compounds, synthesized by Bohlmann. Thus, the Raman technique allows analyses in a very short time replacing the usual time-consuming separation procedures and avoiding artefacts during clean-up procedures. (c) 2004 Elsevier B.V. All rights reserved.
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