Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 293, Issue 1, Pages 382-388Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2005.02.035
Keywords
magnetic tweezers; phage displayed peptide; bond lifetime; ELISA; force measurement; biotin-PEG; streptavidin; protein assay
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A new method has been developed for measuring the binding affinity of phage displayed peptides and a target protein using magnetic particles. The specific interaction between the phage displayed peptides and the target protein was subject to a force generated by the magnetic particle. The binding affinity was obtained by analyzing the force-bond lifetime. (c) 2005 Published by Elsevier B.V.
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