Journal
BRITISH JOURNAL OF PHARMACOLOGY
Volume 145, Issue 2, Pages 246-254Publisher
WILEY
DOI: 10.1038/sj.bjp.0706190
Keywords
reperfusion injury; NF-kappa B; AP-1; TNF-alpha; IL-10
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1 The nuclear translocation of transcription factors may be a critical factor in the intracellular pathway involved in ischaemia/reperfusion (I/R) injury. Here, we examined whether NF-kappa B and AP-1 participated in the cascade of events leading to TNF-alpha production, neutrophil recruitment, tissue injury and lethality following intestinal I/R. 2 The superior mesenteric artery (SMA) of mice was made ischaemic for 60 min followed by 30 min of reperfusion. The effects of NF-kappa B and AP-1 were studied by the administration of the thioredoxin inhibitor, MOL-294 ( methyl 4-hydroxy-4-(8-methyl-1,3-dioxo-2-phenyl-2,3,5,8-tetrahydro- 1H-[1,2,4] triazolo[1,2-a] pyridazin-5-yl) but-2-ynoate), and the AP-1 inhibitor, PNRI-299 (N-benzyl2-(3-cyanophenyl)- 1,3,7-trioxo-2,3,7,8-tetrahydro-1H-[1,2,4] triazolo[1,2-a] pyridazine-5-carboxamide). After I/R, there was increase of translocation of NF-kappa B, but not of AP-1, in the intestine and lungs, as assessed by a gel shift assay. 3 Treatment with MOL-294 inhibited the increase in vascular permeability, neutrophil accumulation, hemorrhage and proinflammatory cytokine levels, induced by intestinal I/R injury in the intestine. In the lungs, MOL-294 partially inhibited edema formation, TNF-alpha production, but did not alter neutrophil recruitment. 4 Treatment with MOL-294 inhibited reperfusion-associated lethality, an effect likely to be secondary to the inhibition of systemic TNF-alpha levels. PNRI-299 had no effects on the inflammatory changes or lethality induced by I/R injury. 5 Our results point to an important role for NF-kappa B in triggering endogenous proinflammatory networks during intestinal I/R injury. Inhibition of NF-kappa B prevents tissue injury and lethality, and this was associated with inhibition of TNF-alpha production and decrease in neutrophil recruitment.
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