Journal
BIOMACROMOLECULES
Volume 6, Issue 3, Pages 1602-1607Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm050016n
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A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O-6-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O-6-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.
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