Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 69, Issue 5, Pages 1036-1039Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.69.1036
Keywords
Bacillus subtilis genome; transformation; inositol; phleomycin; pBR322
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The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
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