Journal
YEAST
Volume 22, Issue 7, Pages 565-569Publisher
WILEY
DOI: 10.1002/yea.1225
Keywords
Saccharomyces cerevisiae; tetracycline-regulatable promoter; doxycycline; essential genes; global gene expression; Affymetrix arrays
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Funding
- Biotechnology and Biological Sciences Research Council [BB/C505140/1] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/C505140/1] Funding Source: researchfish
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The tet-regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is widely accepted that doxycycline is innocuous to yeast. Global genetic studies are now commonplace and the tetO-system is being used in transcriptome studies. Hence, we wanted to ensure that the absence of phenotypic effects, on addition of doxycycline to the growth medium, is mirrored in transcriptome data. We have demonstrated that doxycycline has no significant effect on global transcription levels anti will continue to use the tetO-regulatable promoter system for genetic studies. Copyright (c) 2005 John Wiley & Sons, Ltd.
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