Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 35, Issue 5, Pages 1539-1547Publisher
WILEY
DOI: 10.1002/eji.200425725
Keywords
human; monocytes/macrophages; fungal; cell surface molecules; inflammation
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Funding
- Wellcome Trust Funding Source: Medline
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We identified the C-type-lectin-like receptor, Dectin-1, as the major receptor for fungal beta-glucans on murine macrophages and have demonstrated that it plays a significant role in the cellular response to these carbohydrates. Using two novel, isoform-specific mAb, we show here that human Dectin-1, the P-glucan receptor (PGR), is widely expressed and present on all monocyte populations as well as macrophages, DC, neutrophils and eosinophils. This receptor is also expressed on B cells and a subpopulation of T cells, demonstrating that human Dectin-1 is not myeloid restricted. Both major functional beta GR isoforms - beta GR-A and beta GR-B - were expressed by these cell populations in peripheral blood; however, only beta GR-B was significantly expressed on mature monocyte-derived macrophages and immature DC, suggesting cell-specific control of isoform expression. Inflammatory cells, recruited in vivo using a new skin-window technique, demonstrated that Dectin-1 expression was not significantly modulated on macrophages during inflammation, but is decreased on recruited granulocytes. Despite previous reports detailing the involvement of other beta-glucan receptors on mature human macrophages, we have demonstrated that Dectin-1 acted as the major beta-glucan receptor on these cells and contributed to the inflammatory response to these carbohydrates.
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