Journal
ARCHIVES OF ANDROLOGY
Volume 51, Issue 3, Pages 195-206Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/014850190884372
Keywords
boar sperm; gamete binding; pig; protein sperm; zonapellucida
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This study was conducted to evaluate the role of a 55 kDa pig sperm protein on the oocyte-sperm binding process, and its location in situ. For this purpose, in vitro matured oocytes were incubated with isolated and purified protein, and incubated with capacitated spermatozoa. In addition, capacitated sperm were incubated with anti-55kDa antiserum and later with mature oocyles. Immunolocalization assays were performed using non-capacitated, capacitated and acrosome reacted sperm, which were incubated independently with anti-55 kDa protein antibodies and analyzed under fluorescence light microscopy. The 55 kDa protein concentrations correlated negatively with the amounts of sperm bound to the zona pellucida (ZP); the presence of the anti-55 kDa protein totally inhibited this binding. The immunolocalization assays revealed that fluorescence was located preferentially at the apical edge of the head in capacitated sperm, but not in acrosome reacted sperm. It would appear that the 55 kDa protein binds specifically to the oocyte ZP, and that it may be responsible for primary gamete binding during fertilization.
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