Journal
PARASITOLOGY
Volume 130, Issue -, Pages 489-492Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182004006821
Keywords
Babesia bovis; in vitro cultivation; serum-free medium; drug susceptibility
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Babesia parasites generally require a defined percentage of serum in the culture medium for their in vitro growth. In this study, we attempted to culture Babesia bovis in a serum-free condition. The growth pattern and morphology of B. bovis in serum-free (plain) GIT medium were unaltered as compared to those of the standard growth condition containing 40 % bovine serum in M199. When exposed to the test drugs, the parasite in plain GIT medium showed clearly lower IC50 values than those in 40 % serum-containing GIT medium, indicating that several serum components may interfere with the drug bio-availability. Therefore, the serum-free culture system is useful for standardizing drug test protocols and understanding the roles of serum factors in the drug test.
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