Journal
ANALYTICA CHIMICA ACTA
Volume 538, Issue 1-2, Pages 25-34Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2005.01.058
Keywords
off-line solid-phase microextraction; ibuprofen metabolites; 2-hydroxyibuprofen; carboxyibuprofen; enantiomeric separation; urine
Categories
Ask authors/readers for more resources
A simple, rapid and sensitive off-line solid-phase microextraction method coupled to high-performance liquid chromatography was developed for the stereoselective analysis of the major ibuprofen metabolites in human urine samples. For this purpose, a carbowax/templated/resin coated fiber was used. Several parameters influencing the efficiency of the analyte extraction were explored. The chromatographic separation was achieved on a Chiralpak AS column using hexane:isopropanot (95:5 v/v) plus 0.05% trifluoroacetic acid as the mobile phase, at a flow-rate of 1.2 mL/min. Detection was carried out at 230 nm. The mean recoveries were between 1.4 and 1.6%, with R.S.D. values lower than 9.5 for both 2-OHIbu enantiomers, and between 3.7 and 5.7%, with R.S.D. values lower than 13.4 for all four COOHIbu stereoisomers. The method was linear over the 5-50 mu g/mL concentration range and the quantification limit was 5 mu g/mL for all metabolite stereoisomers. Within-day and between-day assay precision and accuracy for both metabolites were studied at three concentration levels of each stereoisomer (15, 30 and 45 mu g/mL) and were lower than 15%. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available