Journal
JOURNAL OF CELL BIOLOGY
Volume 169, Issue 3, Pages 399-404Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200501121
Keywords
-
Categories
Funding
- NIMH NIH HHS [R56 MH070957, R01 MH070957] Funding Source: Medline
Ask authors/readers for more resources
Dynamic regulation of alpha-amino-3-hydroxy-5-methyl4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immuno-precipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins ( TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope- tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available