Journal
JOURNAL OF CELL BIOLOGY
Volume 169, Issue 3, Pages 491-501Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200407073
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We have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside-(GalNac alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent- resistant membranes ( DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post- Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA W interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM association characteristics of apical proteins. Sulfatides with long chain-hydroxylated fatty acids, which were also enriched in DRMs, were identified as high-affinity ligands for galectin-4. Together, our data propose that interaction between galectin-4 and sulfatides plays a functional role in the clustering of lipid rafts for apical delivery.
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