Fabrication and characterization of human serum albumin and L-α-dimyristoylphosphatidic acid microcapsules based on template technique

Biogenic microcapsules comprised of L-alpha-dimyristoyl phosphatitic acid (DMPA)/human serum albumin (HSA) were prepared by stepwise adsorption of DMPA/HSA multilayers onto charged colloids and subsequent dissolution of the colloidal cores. The properties of both lipid/protein modified particles and the hollow capsules were characterized by microelectrophoresis, scanning electron microscopy, transmission electron microscopy, confocal laser scanning microscopy, and scanning force microscopy, respectively. Single particle light scattering together with small-angle X-ray scattering measurements revealed that DMPA forms a bilayer structure on the HSA surface. A combination of HSA and DMPA on hollow capsules is proposed as a biomimetic membrane model.