Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1075, Issue 1-2, Pages 159-166Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.02.091
Keywords
auxin; abscisic acid; solid phase extraction; 2D-HPLC
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A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in heart cutting mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors. (c) 2005 Elsevier B.V. All rights reserved.
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