Journal
CELL
Volume 121, Issue 4, Pages 529-539Publisher
CELL PRESS
DOI: 10.1016/j.cell.2005.03.009
Keywords
-
Categories
Funding
- NCRR NIH HHS [P41 RR012408] Funding Source: Medline
- NIGMS NIH HHS [R01 GM073863-04, R01 GM070521, R01 GM048410, R01 GM073863-02, R01 GM073863-03, R01 GM 48410, R01 GM073863, R01 GM073863-01, R01 GM 70521] Funding Source: Medline
Ask authors/readers for more resources
The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 angstrom resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available