Journal
FEBS LETTERS
Volume 579, Issue 13, Pages 2811-2816Publisher
WILEY
DOI: 10.1016/j.febslet.2005.04.015
Keywords
ETS; Myeloid Elf-1 like factor; MEF promoter; SP1; gene regulation
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Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5'-flanking region of human MEF gene and identified a TATA-less promoter region at -204/-54 which contains 4 putative binding sites for Sp1, two of which are essential in upregulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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