Journal
VACCINE
Volume 23, Issue 29, Pages 3836-3842Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2005.02.023
Keywords
HIV-1; intranasal immunization; UEA-1
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Receptor-mediated gene transfer using an M cell ligand has been shown to be an efficient method for mucosal DNA immunization. To investigate further into alternative M cell ligands, the plant lectin, Ulex europaeus agglutinin 1 (UEA-1), was tested. UEA-1 binds to human intestinal Caco-2 cells, and these cells can be transfected with poly-L-lysine (PL)-conjugated UEA-1 for expression of reporter cDNAs. When tested in vivo, mice nasally immunized with UEA-1-PL complexed to plasmid encoding HIV-1 envelope showed elevated systemic and mucosal antibody responses, and these were supported by tissue anti body-forming cells. Likewise, elevated envelope-specific CTLs were induced. Thus, UEA-1 mediated DNA delivery represents an alternative mucosal formulation for inducing humoral and cellular immunity against HIV-1. (c) 2005 Elsevier Ltd. All rights reserved.
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