Using 2H2O to study the influence of feeding on protein synthesis:: effect of isotope equilibration in vivo vs. in cell culture

We previously reported that (H2O)-H-2 can be used to measure rates of protein synthesis during prolonged steady-state conditions (Previs SF, Fatica R, Chandramouli V, Alexander JC, Brunengraber H, and Landau BR. Am J Physiol Endocrinol Metab 286: E665-E672, 2004). The underlying premise of our method is that following the administration of 2H2O, H-2 atoms in body water rapidly equilibrate with free alanine before it is incorporated into newly synthesized proteins. We have now directly examined whether (H2O)-H-2 can be used to measure the influence of a single meal on protein synthesis. In addition, we have compared the use of (H2O)-H-2 for measuring rates of protein synthesis in vivo vs. in cell culture. Using a rat model, we observed rapid equilibration between H-2 in body water and free alanine; therefore we were able to study the response of protein synthesis to a single meal. We observed that similar to 50% of the plasma albumin that is synthesized over the course of 24 h is made within similar to 5 h after eating (in rats trained to eat a complete 24-h ration of food in a single meal). Contrary to what we observed in vivo, feeding (the replenishment of cell culture medium) does influence the use of (H2O)-H-2 for in vitro studies. In particular, since there can be slow equilibration of H-2 between water and alanine in the cell culture medium, special consideration must be made to avoid underestimating the rate of protein synthesis in vitro.