Journal
ACTA OPHTHALMOLOGICA SCANDINAVICA
Volume 83, Issue 3, Pages 379-384Publisher
WILEY
DOI: 10.1111/j.1600-0420.2005.00457.x
Keywords
alpha 1-antitrypsin; alpha 1-proteinase inhibitor; total protein; corneal rejection; aqueous humour; ELISA; outcome
Categories
Ask authors/readers for more resources
Purpose: To investigate the presence and concentration of alpha 1-antitrypsin in aqueous humour at the time of corneal rejection and to compare results obtained from patients with reversible and irreversible rejection. Methods: Samples of aqueous humour were obtained from 17 patients with acute corneal endothelial allograft rejection. The presence of alpha 1-antitrypsin in aqueous humour was confirmed by immunoblotting and measured employing a sandwich ELISA. Total protein concentrations in aqueous humour were measured using Bradford's method. The outcome of corneal rejection episodes was determined 1 month after diagnosing corneal rejection and described as reversible or irreversible rejection. Results: alpha 1-antitrypsin was detected in aqueous humour. Patients with reversible rejection had significantly higher alpha 1-antitrypsin concentration than patients with irreversible rejection (p = 0.044). There was no significant difference in total protein concentrations (p = 0.745), and no correlation was found between alpha 1-antitrypsin and total protein concentrations (p = 0.368). Conclusions: alpha 1-antitrypsin in aqueous humour seems to signal a favourable outcome of corneal rejection. The possible mechanism is discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available