Journal
FEMS MICROBIOLOGY LETTERS
Volume 247, Issue 1, Pages 73-79Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.04.028
Keywords
Bacillus subtilis; replication initiation; negative regulator; flow cytometry
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It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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