Journal
ELECTROPHORESIS
Volume 26, Issue 13, Pages 2608-2621Publisher
WILEY
DOI: 10.1002/elps.200410414
Keywords
capillary electrophoresis; laser-induced fluorescence; peptides; proteins; review
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Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (Immunoassay using a labelled antibody or antigen or noncovalent dye interactions), In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF
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