Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 382, Issue 4, Pages 1044-1048Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-3222-4
Keywords
amino-dextran; nanoparticles; gold; silver; concanavalin A; biosensor
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A sensitive method for the detection of the lectin protein concanavalin A (Con A) was developed using amino-dextran (AD)-protected gold (AD-Au) and silver nanoparticles (AD-Ag) as sensitive optical probes. The AD-Au and AD-Ag nanoparticles were synthesized by directly applying amino-dextran as a reductive and protective reagent. The size of the nanoparticles could be altered by changing the molar ratio of AD to the metal salt. The amino-dextran bound to Con A by forming a 4:1 Au-Con A complex at neutral pH, and the nanoparticles were induced to aggregate by Con A. The absorption intensity of the nanoparticles decreased linearly with as the Con A concentration was increased from 3.85x10(-8) to 6.15x10(-7) M. The Au-Con A complex was dissociated by the disaccharide isomaltose, which has a higher affinities for Con A than Au; this competitive strategy could also be used to detect similar types of saccharides.
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