4.5 Article

YB-1 is important for late-stage embryonic development, optimal cellular stress responses, and the prevention of premature senescence

Journal

MOLECULAR AND CELLULAR BIOLOGY
Volume 25, Issue 11, Pages 4625-4637

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.25.11.4625-4637.2005

Keywords

-

Funding

  1. NHLBI NIH HHS [F32 HL077048] Funding Source: Medline
  2. NIDDK NIH HHS [R37 DK038682, DK38682, R56 DK038682] Funding Source: Medline

Ask authors/readers for more resources

Proteins containing cold shock domains belong to the most evolutionarily conserved family of nucleic acid-binding proteins known among bacteria, plants, and animals. One of these proteins, YB-1, is widely expressed throughout development and has been implicated as a cell survival factor that regulates the transcription and/or translation of many cellular growth and death-related genes. For these reasons, YB-1 deficiency has been predicted to be incompatible with cell survival. However, the majority of YB-1(-/-) embryos develop normally up to embryonic day 13.5 (E13.5). After E13.5, YB-1(-/-) embryos exhibit severe growth retardation and progressive mortality, revealing a nonredundant role of YB-1 in late embryonic development. Fibroblasts derived from YB-1(-/-) embryos displayed a normal rate of protein synthesis and minimal alterations in the transcriptome and proteome but demonstrated reduced abilities to respond to oxidative, genotoxic, and oncogene-induced stresses. YB-1(-/-) cells under oxidative stress expressed high levels of the G,specific CDK inhibitors p16Ink4a and p21Cip1 and senesced prematurely; this defect was corrected by knocking down CDK inhibitor levels with specific small interfering RNAs. These data suggest that YB-1 normally represses the transcription of CDK inhibitors, making it an important component of the cellular stress response signaling pathway.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available