Journal
APPLIED CLAY SCIENCE
Volume 95, Issue -, Pages 396-402Publisher
ELSEVIER
DOI: 10.1016/j.clay.2014.05.003
Keywords
Protein adsorption; Montmoiillonite; Langmuir isotherm; Homoionic-clay
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This study aims to investigate the influence of interlayer cation alkali metal series (Li+, Na+, K+, Rb+, Cs+) and alkali earth metal (Mg2+, Ca2+, Ba2+) on adsorption of model proteins (lysozyme and bovine serum albumin). The localizations of both proteins and their conformational modifications in the interlayer space of the natural clay mineral were studied by X-ray diffraction, transmission electronic microscopy and fluorescence experiments. Based on Langmuir model isotherms, a strong influence of interlayer cation on maximum adsorbed amount and adsorption equilibrium constant has been observed. However the usual kosmotrope/chaotrope classification cannot be used to describe protein adsorption. (C) 2014 Elsevier B.V. All rights reserved.
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