Journal
OPTICS LETTERS
Volume 30, Issue 11, Pages 1318-1320Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.30.001318
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01 GM051453, R01 GM057035] Funding Source: Medline
Ask authors/readers for more resources
The focus of an oil-immersion microscope objective is shifted because of the refractive-index mismatch between the cover glass and the aqueous sample. We present a procedure with which to determine the focal shift by use of an inverted microscope equipped with optical tweezers. As the position of the sample chamber is scanned vertically, we measure the axial displacement of an optically trapped bead; the relative motion of the bead with respect to the surface supplies the effective focal shift. Measurements of this quantity deviate from electromagnetic calculations of the focal shift, a discrepancy attributable to the depth-dependent decrease in axial trap stiffness that arises from spherical aberration. (c) 2005 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available