Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 81, Issue 3, Pages 287-300Publisher
SPRINGER
DOI: 10.1007/s11240-004-6648-z
Keywords
Anoectochilus; apple; automated masspropagation; Chrysanthemum; dissolved oxygen; garlic; ginseng; grape; Lilium; micropropagation; mixing; nutrients; pH; Phalaenopsis; potato; secondary metabolites; siberian ginseng; somatic embryogenesis
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Automation of micropropagation via organogenesis or somatic embryogenesis in a bioreactor has been advanced as a possible way of reducing costs. Micropropagation by conventional techniques is typically a labour-intensive means of clonal propagation. The paper describes lower cost and less labour-intensive clonal propagation through the use of modified air-lift, bubble column, bioreactors (a balloon-type bubble bioreactor), together with temporary immersion systems for the propagation of shoots, bud-clusters and somatic embryos. Propagation of Anoectochilus, apple, Chrysanthemum, garlic, ginseng, grape, Lilium, Phalaenopsis and potato is described. In this chapter, features of bioreactors and bioreactor process design specifically for automated mass propagation of several plant crops are described, and recent research aimed at maximizing automation of the bioreactor production process is highlighted.
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