Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 43, Issue 6, Pages 2944-2947Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.43.6.2944-2947.2005
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We have recently described a novel AB(5) subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx(1) and str(2). The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.
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