Journal
DEVELOPMENT
Volume 132, Issue 11, Pages 2669-2678Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.01849
Keywords
cardiac gene expression; skeletal muscle gene; expression; MEF2 binding site; E-box; cardiogenesis; mouse; anterior heart field
Categories
Funding
- NHLBI NIH HHS [HL074663, HL71160] Funding Source: Medline
Ask authors/readers for more resources
The vertebrate heart is assembled during embryogenesis in a modular manner from different populations of precursor cells. The right ventricular chamber and outflow tract are derived primarily from a population of progenitors known as the anterior heart field. These regions of the heart are severely hypoplastic in mutant mice lacking the myocyte enhancer factor 2C (MEF2C) and BOP transcription factors, suggesting that these cardiogenic regulatory factors may act in a common pathway for development of the anterior heart field and its derivatives. We show that Bop expression in the developing heart depends on the direct binding of MEF2C to a MEF2-response element in the Bop promoter that is necessary and sufficient to recapitulate endogenous Bop expression in the anterior heart field and its cardiac derivatives during mouse development. The Bop promoter also directs transcription in the skeletal muscle lineage, but only cardiac expression is dependent on MEF2. These findings identify Bop as an essential downstream effector gene of MEF2C in the developing heart, and reveal a transcriptional cascade involved in development of the anterior heart field and its derivatives.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available