Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 71, Issue 6, Pages 3342-3347Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.71.6.3342-3347.2005
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Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.
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