Journal
MOLECULAR ECOLOGY NOTES
Volume 5, Issue 2, Pages 259-262Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1471-8286.2005.00903.x
Keywords
5'-nuclease assay; chum salmon; Oncorhynchus keta; PCR; SNP; TaqMan
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We report the characterization of 13 single nucleotide polymorphism (SNP) genotyping assays for chum salmon (Oncorhynchus keta). These assays are based on the 5'-nuclease reaction and thus facilitate high-throughput genotyping with minimal optimization time. Because data generated using these markers may be transported and combined across laboratories, SNPs offer the potential to reduce the amount of redundant work being done in mixture and migratory studies of chum salmon.
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