Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume 1734, Issue 3, Pages 277-288Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2005.04.003
Keywords
uptake; phospholipid; TAG; beta-oxidation; secretion; salmon
Ask authors/readers for more resources
Isolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven 14 C-labelled fatty acids. The fatty acids were [1-C-14]16:0, [1-C-14]18:1n-9, 91-C-14]18:2n-6, [1-C-14] 18:3n-3, [1-C-14]20:4n-6, [1-C-14]20:5n-3, and [1-C-14]22:6n-3. After 2 It of incubation, the hepatocytes and medium were analysed for acid soluble products, incorporation into lipid classes, and hepatocytes for desaturation and elongation. Uptake into hepatocytes was highest with [1-C-14]18:2n-6 and [1-C-14]20:5n-3 and lowest with [1-C-14]16:0. The highest recovery of radioactivity in the cells was found in triacylglycerols. Of the phospholipids, the highest recovery was found in phosphatidylcholine, with [1-C-14] 16:0 and [1-C-14]22:6n-3 being the most prominent fatty acids. The rates of beta-oxidation were as follows: 20:4n-6 > 18:2n-6= 16:0 > 18:1n-9 > 22:6n-3=18:3n-3=20:5n-3. Of the fatty acids taken up by the hepatocytes, [1-C-14]16:0 and [1-C-14]18:1n-9 were subsequently exported the most, with the majority of radioactivity recovered in phospholipids and triacylglycerols, respectively. The major products from desaturation and elongation were generally one cycle of elongation of the fatty acids. Diet had a clear effect on the overall lipid metabolism, with replacing 75% of the fish oil with vegetable oil resulting in decreased uptake of all fatty acids and reduced incorporation of fatty acids into cellular lipids, but increased beta-oxidation activity and higher recovery in products of desaturation and elongation of[1-C-14]18:2n-6 and [1-C-14] 18:3n-3. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available