Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 123, Issue 6, Pages 896-899Publisher
OXFORD UNIV PRESS INC
DOI: 10.1309/MPERNJ0G62RECHCQ
Keywords
human papillomavirus; detection; typing
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Detection and typing of human papillomaviruses (HPVs) are requested by clinicians with growing frequency as part of the overall management of patients. A new method using consensus L1 amplification and a reverse line blot hybridization detection method has been described for broad spectrum HPV genotype identification. This method involves hybridization at 53 degrees C in a shaking water bath, which many laboratories might find cumbersome. We describe a modified hybridization step, using a dry-air incubator, that simplifies the detection protocol. Overall, comparable results were obtained by the modified, more easily performed protocol, indicating that laboratories might validate and use this modified method.
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