Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters

Background: Toxic lipid peroxides are known to cause various impairments of sperm cells and may play a major role in the etiology of male infertility. We determined the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospennic males. Methods: NIDA of spermatozoa and seminal plasma was determined in 35 asthenozoospermic and 15 normozoospermic men by spectrofluorometry. Semen analysis was done according to the WHO standard. Results: MDA concentration in the spermatozoa of asthenozoospermic was significantly higher than in normozoospermic males (0.14 +/- 0.059 and 0.09 +/- 0.04 nmol/10 x 10(6) spermatozoa respectively). The mean +/- S.D. MDA value in the seminal plasma of asthenozoospermic and non-nozoospermic were 1.35 +/- 0.42 and 1.2 +/- 0.3 nmol/ml seminal plasma respectively. Conclusions: Lipid peroxidation has a deleterious effect on the semen quality and MDA is an index of lipid peroxidation which may be a diagnostic tool for the analysis of infertility in the asthenozoospermic patients. (c) 2005 Elsevier B.V. All rights reserved.